The Alber Lab
OSU MicrobiologyThe PeopleMutant StrainsSelected Publications
Molecular Genetics classes in the Microbiology Department at the Ohio State University (taught by Kathleen Sandman) generated more than 25,000 random transposon mutants of Rhodobacter sphaeroides strain 2.4.1 that grow on a defined minimal medium with either succinate or D/L-lactate as the carbon source.  The work was presented by Emily Smith and Jordan Allen at the 2010 Denman Undergraduate Research Forum.  A copy of their poster is available here.

Microbial Genetics Labs

Microbial Genetics Labs 2

Funding By:

 NSF

Minimal medium composition (1 L): 22.4 mM NH4Cl, 0.8 mM MgSO4, 0.5 mM CaCl2, 15 mM potassium phosphate buffer, pH 6.7, 1 ml/1 liter vitamin solution (100 mg cyanocobalamin, 300 mg pyridoxamine-2HCl, 100 mg calcium-D(+)-pantothenate, 200 mg thiamindichloride, 200 mg nicotinic acid, 80 mg 4-aminobenzoic acid, 20 mg D(+)-biotin), 10 ml/liter trace element solution (500 mg ethylenediamine tetraacetic acid (EDTA, disodium salt), 300 mg FeSO4 7H2O, 3 mg MnCl2 4H2O, 5 mg CoCl2 6H2O, 1 mg CuCl2 2H2O, 2 mg NiCl2 6H2O, 3 mg Na2MoO4 2H2O, 5 mg ZnSO4 7H2O, and 2 mg H3BO3 per liter, pH 3) and 10 mM of either sodium succinate or sodium lactate.

The following table, assembled by Jordan Allen and Birgit Alber, lists those mutants for which the transposon insertion has been mapped and a stock culture is available upon request.  Please contact Birgit Alber (alber.8@osu.edu) with any requests for stock cultures.


This table is also available as an Excel spreadsheet here.

Mutant Minimal Media Mutated Gene/Intergenic Region Sequence insertion Site Chromosome/Plasmid Tn Location RSP Primer Date Obtained1 Current Box Position
Sp11-32-212 D/L lactate hypothetical protein (0056536-0056544) chromosome 1 1471 13-2, 17-1 6/23/2011 P Box2 I7
SP10-29-4 D/L lactate oligopeptide transporter, fused ATPase subunits 0063798  chromosome 1 1478 17-1 5/27/2010 P Box1 A1
Au10TR-25-35 succinate GAF domain-containing protein 0088228 chromosome 1 1502 17-1 11/30/2010 P Box2 E4
Au12-332-11-45 D/L lactate ribosomal large subunit pseudouridine synthase A 0089655 chromosome 1 1506 17-1 12/13/2012 P Box3 C3
Au10MW-21-65 succinate HNS family histone-like protein 0104180 chromosome 1 1517 17-1 11/30/2010 P Box2 G2
Au12-316-7-pig D/L lactate PrrA (RegA), response regulator involved in oxygen regulation of photosynthesis genes 0105027 chromosome 1 1518 17-1 12/13/2012 P Box3 G4
Au10TR-22-88 succinate transthyretin-like protein 0146035 chromosome 1 1555 17-1 11/30/2010 P Box2 C5
Au12-316-14-141(pig) D/L lactate AppA, BLUF, antirepressor of ppsR, sensor of blue light 0155442 chromosome 1 1565 17-1 12/13/2012 P Box3 F4
Au12-316-17-251(pig) D/L lactate AppA, BLUF, antirepressor of ppsR, sensor of blue light 0156313 chromosome 1 1565 17-1 12/13/2012 P Box3 A4
Sp11-21-87 D/L lactate Chemotaxis protein, CheW2 0176815 chromosome 1 1585 17-1 6/23/2011 P Box2 F6
Au12-332-17-124 D/L lactate Chemotaxis protein, CheW2 0176853 chromosome 1 1585 17-1 12/13/2012 P Box1 D3
Sp11-35-40 D/L lactate hypothetical protein 0195032 chromosome 1 6009 17-1 6/23/2011 P Box2 G6
Sp10-18-MF D/L lactate conserved hypothetical protein 0241614-0241624 chromosome 1 1648 13-2, 17-1 5/27/2010 P Box3 A1
Au10MW-29-11 succinate hypothetical protein 0248309 chromosome 1 1654 17-1 11/30/2010 P Box2 C2
Au11-30-101 D/L lactate RelA/SpoT family protein 0260844 chromosome 1 1670 17-1 12/12/2011 P Box3 A1
Au12-332-18-247 D/L lactate RelA/SpoT family protein 0260932 chromosome 1 1670 17-1 12/13/2012 P Box3 G3
Au12-332-1-132 D/L lactate RelA/SpoT family protein 0261677 chromosome 1 1670 17-1 12/13/2012 P Box3 H3
Au11-24-50 D/L lactate n/a 0275521 (estimate) chromosoma 1 n/a iPCR 12/12/2011 P Box3 G1
SP10-11-A3 D/L lactate possible acyltransferase (365 aa) 0277757-277766 chromosome 1 1688 13-2, 17-1 5/27/2010 P Box1 C1
Au12-316-3-pig D/L lactate putative transcriptional regulatory protein (LacI family) 0370075 chromosome 1 1785 17-1 12/13/2012 P Box3 D5
Sp11-28-9 D/L lactate hypothetical protein 0393762-0393771 chromosome 1 6019 13-2, 17-1 6/13/2011 P Box2 H6
Au09MW-2-89 succinate Mg chetalase-related protein 0406029  chromosome 1 1539 13-2 11/30/2009 P Box1 D1
Sp11-6-87 D/L lactate Zinc-containing alcohol dehydrogenase 0415903 chromosome 1 1824 17-1 6/23/2011 P Box2 E5
Au10TR-14-142 succinate TPR repeat-containing protein 0500304 chromosome 1 1902 17-1 11/30/2010 P Box2 A4
SP10-33-36 D/L lactate intergenic region between AsnC family transcriptional regulator and cytochrome P450 hydroxylase 0543649-543659 chromosome 1 n/a 13-2, 17-1 5/27/2010 P Box1 E1
Au10TR-02-03 succinate hypothetical protein 0554801 chromosome 1 1962 17-1 11/30/2010 P Box2 B4
Au09TR-09-40 (=Au09MW-09-42) succinate intergenic region between ATPase and hypothetical protein 0639371-639383 chromosome 1 2044-2045 13-2, 17-1 11/30/2009 P Box1 F1
Au09MW-09-42 (=Au09TR-09-40) succinate intergenic region between ATPase and hypothetical protein 0639371-639383 chromosome 1 2044-2045 13-2, 17-1 11/30/2009 P Box1 G1
SP10-7-4 D/L lactate hypothetical protein 0644741  chromosome 1 2049 17-1 05/27/2010 P Box1 H1
SP10-19-3 D/L lactate conserved hypothetical protein 0651428  chromosome 1 2058 17-1 05/27/2010 P Box1 I1
Au09MW-28-52 succinate pyruvate carboxylase (PycA) 0685258-685267 chromosome 1 2090 13-2, 17-1 11/30/2009 P Box1 A2
Au09TR-23-48 succinate pyruvate carboxylase (PycA) 0685308-685319 chromosome 1 2090 13-2, 17-1 11/30/2009 P Box1 B2
Au09TR-32-90 succinate pyruvate carboxylase (PycA) 0685750 (estimate) chromosome 1 2090 iPCR 11/30/2009 P Box1 C2
Au09TR-09-187 succinate pyruvate carboxylase (PycA) 0686061-686069 chromosome 1 2090 13-2, 17-1 11/30/2009 P Box1 D2
Au09TR-17-143 succinate pyruvate carboxylase (PycA) 0686692-686701 chromosome 1 2090 13-2, 17-1 11/30/2009 P Box1 E2
Au09TR-34-134 succinate pyruvate carboxylase (PycA) 0686932-686944 chromosome 1 0290 13-2, 17-1 11/30/2009 P Box1 F2
Au09MW-33-186 succinate small conductance mechanosensitive ion channel 0750915 (estimate) chromosome 1 2148 iPCR 11/30/2009 P Box1 G2
Au09MW-19-44 succinate major facilitator superfamily multidrug-efflux transporter 0795368-795376 chromosome 1 2187 13-2, 17-1 11/30/2009 P Box1 H2
Au09TR-39-86 succinate hypothetical protein 0801678-801687 chromosome 1 6041 13-2, 17-1 11/30/2009 P Box1 I2
Au11-44-29 D/L lactate FadJ enoyl-CoA hydratase/3-OHacyl DH/3-OHbutyryl-CoA epimerase 0810169 chromosome 1 2196 17-1 12/12/2011 P Box2 D9
Sp11-28-160 D/L lactate MerR family transcriptional regulator 0815944-0815956 chromosome 1 2201 13-2, 17-1 6/13/2011 P Box2 I6
Au09MW-20-156 succinate hypothetical protein 0852943 (estimate) chromosome 1 2235 iPCR 11/30/2009 P Box1 A3
SP10-7-11 D/L lactate long chain fatty acid-CoA ligase (629 aa) 0873893-873901 chromosome 1 2255 13-2, 17-1 5/27/2010 P Box1 C3
SP10-7-21 D/L lactate antifreeze protein, type 1 (ydjI; 369 aa) 0878371-878380 chromosome 1 2261 13-2, 17-1 5/27/2010 P Box1 B3
Au12-316-15-96 D/L lactate putative anaerobic phenyllactate CoA ligase 0891467 chromosome 1 2274 17-1 12/13/2012 P Box3 D4
Au10TR-8-105 succinate branched chain amino acid ABC transporter ATPase 0892956 chromosome 1 2274 17-1 11/30/2010 P Box2 C4
Au11-40-189 D/L lactate putative ATP-helicase, MgpS 1052712 chromosome 1 2422 17-1 12/12/2011 P Box2 G9
Au11-39-200 D/L lactate (glutathione-reg. K+ efflux protein KefB) 1059208 (estimate) chromosome 1 n/a iPCR 12/12/2011 P Box2 H9
Au10TR-3-57 succinate glutathione-dependent formaldehyde-activating enzyme 1219729 chromosome 1 2575 17-1 11/30/2010 P Box2 C1
Au12-332-8-pig D/L lactate xoxF methanol dehydrogenase 1222638 chromosome 1 2578 17-1 12/13/2012 P Box1 F6
SP10-11-81 D/L lactate intergenic region between hypothetical proteins 1411640-1411649 chromosome 1 2765-6070 13-2, 17-1 05/27/2010 P Box1 E3
Au12-332-5-p D/L lactate multidrug/cation efflux pump, RND superfamily 1514717 chromosome 1 2803 17-1 12/13/2012 P Box1 A7
Au10TR-34-110 succinate tellurite resistance protein 1537079 chromosome 1 2869 17-1 11/30/2010 P Box2 D4
Au09MW-22-193 succinate putative site-specific recombinase 1705707 (estimate) chromosome 1 0003 iPCR 11/30/2009 P Box1 F3
Au12-316-20-95 D/L lactate intergenic 1720793 chromosme 1 n/a 17-1 12/13/2012 P Box3 A5
SP10-15-122 D/L lactate hypothetical protein (263 aa) 1724183-1724191 chromosome 1 6083 13-2, 17-1 05/27/2010 P Box1 G3
Au10TR-30-84 succinate NAD(P)-dependent oxidoreductase 1728103 chromosome 1 0025 17-1 11/30/2010 P Box2 G1
Au10-18-13 succinate n/a 1733582 (estimate) chromosome 1 n/a iPCR 11/30/2010 P Box3 H2
Au10TR-33-43 succinate sigma-54 dependent trancsriptional regulator 1779096 chromosome 1 0071 17-1 11/30/2010 P Box2 H3
Au09TR-35-134 succinate sigma 54 dependent transcriptional regulator, fleQ, flagellum synthesis regulation 1779895 (estimate) chromosome 1 0071 iPCR 11/30/2009 P Box1 H3
SP10-5-17 (=SP10-OZ69A) D/L lactate hypothetical protein (235 aa) 1852875-1852889 chromosome 1 0137 13-2, 17-1 5/27/2010 P Box1 I3
Au09TR-2-47 succinate signal transduction histidine kinase 1861895-1861904 chromosome 1 0148 13-2, 17-1 11/30/2009 P Box1 A4
Sp11-KS40 D/L lactate NAD-dep. epimerase/dehydratase 1876555-1876563 chromosome1 0160 13-2, 17-1 8/11/2011 P Box3 B3
Sp11-3-37 D/L lactate NAD-dep. epimerase/dehydratase 1876898 chromosome 1 0160 17-1 6/23/2011 P Box2 D5
SP10-4-15 D/L lactate lactate dehydrogenase and related DHs 1891783  chromosome 1 175 17-1 5/27/2010 P Box1 B4
Au10MW-6-color2 succinate dihydroxy-acid dehydratase 1893529 chromosome 1 0176 17-1 11/30/2010 P Box2 F2
Sp11-30-154 (pig) D/L lactate phage-related integrase 1930396 chromosome 1 0217 17-1 6/23/2011 P Box2 G5
Sp11-36-100 D/L lactate plasma membrane H+-transporting 2-sector ATPase, C subunit 1936924-1936933 chromosome 1 0221 13-2, 17-1 6/13/2011 P Box A7
Au09TR12-109 succinate plasma membrane H+-transporting 2-sector ATPase, C subunit 1937926-1937938 chromosome 1 0221 13-2, 17-1 11/30/2009 P Box1 C4
Au11-22-44 D/L lactate n/a 1938066 (estimate) chromosome 1 n/a iPCR 12/12/2011 P Box3 E1
Au11-11-04 D/L lactate n/a 1940305 (estimate) chromosome 1 n/a iPCR 12/12/2011 P Box3 F2
Au10MW-18-28 succinate protein pufQ 1984583 chromosome 1 0259 17-1 11/30/2010 P Box2 D1
Au10TR-15-63 succinate putative chlorophyllide reductase, BchZ subunit 1986173 chromosome 1 0260 17-1 11/30/2010 P Box2 I1
Au12-316-5-50 D/L lactate crtE, geranylgeranyl pyrophosphate synthetase (very beginning) 1989676 chromosome 1 0265 17-1 12/13/2012 P Box2 B4
Au11-5-198 D/L lactate farnesyl-diphosphate synthase (ctrE) 1989967 chromosome 1 0265 17-1 12/12/2011 P Box3 D2
SP10-32-10 D/L lactate methoxyneurosporene dehydrogenase (crtD; 495 aa) 1990714-1990723 chromosome 1 0266 13-2, 17-1 5/27/2010 P Box1 D4
Sp11-28-26 (pig) = Au11-47-183 D/L lactate methoxyneurosporene dehydrogenase (crtD; 495 aa) 1991751 chromosome 1 0266 17-1 6/13/2011 P Box2 A3 and              P Box3 I9
SP10-MA6-1 D/L lactate methoxyneurosporene dehydrogenase (crtD; 495 aa) 1991957-1991966 chromosome 1 0266 13-2, 17-1 5/27/2010 P Box1 E4
Au11-16-132 (pig) D/L lactate (ctrI, phytoene dehydrogenase) 1995219 (<, estimate) chromosome 1 n/a iPCR 12/12/2011 P Box3 B2
Au09TR-31 (pig) succinate spheroidene monooxygenase, carotenoid biosynthesis 1997193 chromosome 1 0272 13-2 11/30/2009 P Box1 F4
Au12-316-5-pig D/L lactate ctrA, spheroidene monooxygenase 1997331 chromosome 1 0272 17-1 12/13/2012 P Box3 C4
Au12-316-19-pig D/L lactate bchG, putative bacteriochlorophyll synthase 2004915 chromosome 1 0279 17-1 12/13/2012 P Box3 F5
Au12-316-23-pig D/L lactate bchF, 2-vinyl bacteriochlorophyllide hydratase 2010703 chromosome 1 0284 17-1 12/13/2012 P Box3 E4
Au10MW-5-1 (pig) succinate magnesium chelatase subunit H 2014014 chromosome 1 0287 17-1 11/30/2010 P Box2 F1
SP10-28-6 (pig) D/L lactate hydrogeobyrinic acid a,c-diamide cobalt chetalase (bchH; 1193 aa) 2014487-2014496 chromosome 1 0287 13-2, 17-1 5/27/2010 P Box1 G4
Au12-316-16-235 D/L lactate puhA reaction center H protein 2021393 chromosome 1 0291 17-1 12/13/2012 P Box3 E5
Sp11-16-91 D/L lactate homoserine kinase protein 2040713-2040721 chromosome 1 0311 13-2, 17-1 6/13/2011 P Box2 D8
Au11-32-93 D/L lactate intergenic 2043166 chromosome 1 n/a 17-1 12/12/2011 P Box3 D1
Au11-20-222 D/L lactate (pucA, LHIIalpha light harvesting) 2042953 (>, estimate) chromosome 1 n/a iPCR 12/12/2011 P Box2 C2
Au11-19-231 (pig) D/L lactate PucA, LHII, light harvesting B800/850 2043259 chromosome 1 6356 17-1 12/12/2011 P Box3 C1
Au10MW-22-pig succinate RarD family protein 2093369 chromosome 1 0364 17-1 11/30/2010 P Box2 E1
Au12-316-25-208 D/L lactate hypothetical protein 2149827 chromosome 1 0414 17-1 12/13/2012 P Box3 I5
Au10TR-10-51 succinate isocitrate dehydrogenase 2177435 chromosome 1 0446 17-1 11/30/2010 P Box2 E3
SP10-MA8-34 D/L lactate transaldolase (219 aa) 2299749-2299767 chromosome 1 0561 13-2, 17-1 5/27/2010 P Box1 H4
SP10-BA201 D/L lactate CDP-diacylglycerol-choline O-phosphatidyltransferase (238 aa) and intergenic region (+3 bp) 2303048-2303059 chromosome 1 0565 13-2, 17-1 5/27/2010 P Box1 I4
Au11-50-207 D/L lactate putative exonuclease 2312658 chromosome 1 0572 17-1 12/12/2011 P Box2 F9
Au10TR-06-PG2 succinate RNA polymerase sigma factor SigJ 2348360 chromosome 1 0607 17-1 11/30/2010 P Box2 H1
Sp11-15-74 D/L lactate RhtB family transporter 2351369-2351371 chromosome 1 0612 13-5, 17-1 6/13/2011 P Box 2 I8
SP10-MA6-5 D/L lactate NUDIX hydrolase 2453344-2453353 chromosome 1 0708 13-2, 17-1 05/27/2010 P Box1 A5
Au10TR-29-33 succinate hypothetical protein 2459108 chromosome 1 0713 17-1 11/30/2010 P Box2 I4
Sp11-32-40 D/L lactate hypothetical protein 2477621 chromosome 1 0732 17-1 6/13/2011 P Box2 H7
Sp11-12-113 D/L lactate adenine-specific DNA methylase 2491953-2491964 chromosome 1 0748 13-2, 17-1 7/11/2011 P Box2 E8
Au11-36-71 D/L lactate esterase/lipase/thioesterase 2567068 chromosome 1 0821 17-1 12/12/2011 P Box3 B1
Au09TR-12-79 succinate hypothetical protein (next to putative flavoprotein) 2582572-2582582 chromosome 1 6136 13-2, 17-1 11/30/2009 P Box1 B5
Au09MW-6-13 succinate putative transporter, Rha T family, DMT superfamily 2627431  chromosome 1 0877 13-2 11/30/2009 P Box1 C5
Au09TR-07-35 succinate catalase 2653252-2653265 chromosome 1 0903 13-2, 17-1 11/30/2009 P Box1 B1
Au10-16-64 succinate hypothetical protein 2705950 chromosome 1 0953   11/30/2010 p Box3 A3
Sp11-22-112 D/L lactate Na+/H+ antiporter, NhaD 2756911-2756903 chromosome 1 0998 13-2, 17-1 6/13/2011 P Box2 C9
Au12-316-4-PM D/L lactate conserved hypothetical protein 2785256 chromosome 1 1022 17-1 12/13/2012 P Box3 I3
Sp11-3-144 D/L lactate Peptidase family S49 2832475-2832466 chromosome 1 1076 13-2, 17-1 8/9/2011 P Box2 A9
Au09TR-19-157 succinate sugar/cation symporter, GPH family 2846581 (estimate) chromosome 1 1089 iPCR 11/30/2009 P Box1 H5
Au12-332-11-180 D/L lactate 4Fe-4S ferredoxin, iron-sulfur cluster binding protein 2904887 chromosome 1 1143 17-1 12/13/2012 P Box3 F3
Au10TR-1-27 succinate NADH-ubiquinone oxidoreductase 2915309 chromosome 1 1151 17-1 11/30/2010 P Box2 G4
Au09T18-151 succinate n/a 2931863 (estimate) chromosome 1 1167 iPCR 11/30/2009 P Box1 D5
Au10MW-31-79 succinate prolyl aminopeptidase 2942582 chromosome 1 1174 17-1 11/30/2010 P Box2 A3
Au09MW19-42 succinate secreted conserved hypothetical protein 2970001-2970010 chromosome 1 1199 13-2, 17-1 11/30/2009 P Box1 E5
Au10TR-5-118 succinate NUDIX hydrolase 2979607 chromosome 1 1208 17-1 11/30/2010 P Box2 G3
SP10-18-BA D/L lactate ABC sugar transporter, ATPase subunit (499 aa) 3016062-3016071 chromosome 1 1247 13-2, 17-1 5/27/2010 P Box1 F5
Au10MW-34-9 succinate hypothetical protein 3072968 chromosome 1 6154 17-1 11/30/2010 P Box2 C3
Sp11-10-59 D/L lactate putative MotB protein 3073686-3073687 chromosome 1 1302 13-2, 17-1 6/11/2011 P Box2 F8
Au12-316-20-9 D/L lactate flgE, putative flagellar hook protein 3074884 chromosome 1 1303 17-1 12/13/2012 P Box3 B5
Au09TR-30-024 succinate hypothetical protein, gene cluster with flagellum biosynthesis genes 3090075 chromosome 1 1318 13-2 11/30/2009 P Box1 G5
Sp11-35-169 D/L lactate intergenic region 3102834-3102842 chromosome 1 n/a 13-2, 17-1 9/8/2011 P Box2 I8
SP10-22-4 D/L lactate transcriptional regulator, TetR family (233 aa) 0053600-53610 chromosome 2 3022 13-2, 17-1 5/27/2010 P Box1 I5
Au09MW-01-229 succinate molybdenum cofactor biosynthesis protein (moeA) 0086469-86477 chromosome 2 3049 13-2, 17-1 11/30/2009 P Box1 A6
SP10-26-1 (pig) D/L lactate periplasmic sensor diguanylate cyclase/phosphodiesterase 0145334 chromosome 2 3101 17-1 05/27/2010 P Box1 C6
Au09MW-12-91 succinate putative succinate dehydrogenase/fumarate reductase, flavoprotein subunit 0197684  chromosome 2 3147 13-2 11/30/2009 P Box1 D6
Au10TR-12-1 (green) succinate ABC ferric siderophore transporter, inner membrane subunit 0279538 chromosome 2 3220 17-1 11/30/2010 P Box2 A2
SP10-29-5 D/L lactate NAD-dependent deacetylase (230 aa) 0285267-285276 chromosome 2 3226 13-2, 17-1 5/27/2010 P Box1 F6
Au09MW-15-23 succinate L-lysine 2,3-aminomutase 0286543-286552 chromosome 2 3227 13-2, 17-1 11/30/2009 P Box1 G6
Au11-18-41 D/L lactate ABC peptide transporter, periplasmic binding protein 0289451 chromosome 2 3231 17-1 12/12/2011 P Box3 E2
Sp11-2-87 D/L lactate acrB, cation/multidrug efflux pump 0379697 chromosome 2 3318 17-1 6/23/2011 P Box2 E6
Sp11-5-119 D/L lactate transporter RarD family protein 0405046-0405055 chromosome2 3341 13-2, 17-1 8/9/2011 P Box2 G8
Sp11-11-192 D/L lactate methionine synthase, 5-methyltetrahydrofolate-homocysteine methyltransferase 0412665 chromosome 2 3346 17-1 6/23/2011 P Box2 F5
Au10MW-19-60 succinate exodeoxyribonuclease III 0491929 chromosome 2 3423 17-1 11/30/2010 P Box2 D2
Sp11-36-144 D/L lactate transcriptional regulator 0519098-519090 chromosome 2 3448 13-1, 17-1 6/13/2011 P Box2 G7
Sp11-4-150 D/L lactate protein phosphatase 2C-like 0568134 chromosome 2 3492 17-1 6/23/2011 P Box2 A6
Au10TR-35-2 succinate acetamidase/formamidase 0587818 chromosome 2 3508 17-1 11/30/2010 P Box2 A5
SP10-7-41 (=SP10-8-37) D/L lactate putative adhesin or RTX toxin (2040 aa) 0627995-628004 chromosome 2 3540 13-2, 17-1 5/27/2010 P Box1 H6
Au10MW-33-230 succinate dimethylglycine dehydrogenase 0661547 chromosome 2 3566 17-1 11/30/2010 P Box2 B3
Au11-31-63 D/L lactate ZnuA, ABC zinc transporter, periplasmic binding protein 0666948 chromosome 2 3571 17-1 12/12/2011 P Box3 A2
SP10-KL15-39 D/L lactate hypothetical protein (108 aa) 0743017-743025 chromosome 2 3631 13-2, 17-1 5/27/2010 P Box1 I6
SP10-5-39 D/L lactate putative ExsB (207 aa) 0750649-750657 chromosome 2 3642 13-2, 17-1 5/27/2010 P Box1 B7
Au12-316-13-5 D/L lactate metal dependent phosphohydrolase 0751996 chromosome 2 3644 17-1 12/13/2012 P Box3 I4
Au10TR-31-14 succinate metal dependent phosphohydrolase 0752175 chromosome 2 3644 17-1 11/30/2010 P Box2 B5
Au10TR-29-51 succinate metal dependent phosphohydrolase 0752189-752198 chromosome 2 3644 13-2, 17-1 11/30/2010 P Box1 E6
Au09TR-25-180 succinate n/a 0753439 (estimate) chromosome 2 n/a iPCR 11/30/2009 P Box1 D7
Au11-37-223 D/L lactate hypothetical protein 0753589 chromosome 2 3645 17-1 12/12/2011 P Box3 F1
Au11-08-11 D/L lactate n/a 0753870 (estimate) chromosome 2 n/a iPCR 12/12/2011 P Box3 H1
Sp11-10-92 D/L lactate intergenic region 0755976 chromosome 2 n/a 17-1 6/23/2011 P Box2 B6
Au09MW-17-125 succinate hypothetical protein 0786211  chromosome 2 3669 13-2 11/30/2009 P Box1 E7
Sp11-34-64 D/L lactate hypothetical protein 0787413-0787404 chromosome 2 3669 13-2, 17-1 8/9/2011 P Box2 B1
Sp11-22-13 D/L lactate ABC sulfate/thiosulfate transporter, inner membrane subunit CysW 0820837-0820849 chromosome 2 3698 13-2, 17-1 7/8/2011 P Box2 H8
Au09MW-20-57 succinate methyl-accepting chemotaxis protein (mcpJ) 0831545-831554 chromosome 2 3708 13-2, 17-1 11/30/2009 P Box1 F7
Au12-332-14-191 D/L lactate capsular polysaccharide synthesis protein 0842946 chromosme 2 3719 17-1 12/13/2012 P Box3 E3
Au09MW11-15 succinate hypothetical protein (569 aa) 0851788-851796 chromosome 2 3725 13-2, 17-1 11/30/2009 P Box1 G7
Au09MW-27-59 succinate hypothetical protein 0876048-876056 chromosome 2 3750 13-2, 17-1 11/30/2009 P Box1 H7
Au12-316-25-pig D/L lactate hypothetical protein 0880724 chromosome 2 3754 17-1 12/13/2012 P Box H5
Sp11-34-241 D/L lactate hypothetical protein 0882524 chromosome 2 3756 17-1 6/23/2011 P Box2 C6
Au10TR-43-11 succinate hypothetical protein 0917601 chromosome 2 3797 17-1 11/30/2010 P Box2 I3
Au11-51-166 D/L lactate TRAP-T family transporter 0935735 chromosome 2 1418 17-1 12/12/2011 P Box2 E9
SP10-OZ69 D/L lactate predicted O-linked acetylglucosamine transferase 0019132-19123 plasmid A 3853 13-2, 17-1 5/27/2010 P Box1 A9
Au11-26-39 D/L lactate predicted O-linked acetylglucosamine transferase 0019730 plasmid A 3835 17-1 12/12/2011 P Box3 G2
Au09MW-22-44 succinate cysA 0030861-30870 plasmid A 3858 13-2, 17-1 11/30/2009 P Box1 C8
Au10MW-4-2 (green) succinate sulfate/molybdate ABC transporter inner membrane protein 0032305 plasmid A 3860 17-1 11/30/2010 P Box2 E2
Au09TR-16-42 succinate sulfate/molybdate ABC transporter inner membrane protein 0032775-32786 plasmid A 3860 13-2, 17-1 11/30/2009 P Box1 D8
Au10MW-32-73 succinate BadM/Rrf2 family transcriptional regulator 0035277 plasmid A 3865 17-1 11/30/2010 P Box2 H2
SP10-32-08 D/L lactate modA, ABC molybdate transporter, periplasmic binding protein modA 0041751  plasmid A 3871 17-1 05/27/2010 P Box1 E8
SP10-4-36 D/L lactate modD, quinolinate phosphoribosyl transferase/putativemolybdenum utilization protein ModD 0043275  plasmid A 3873 17-1 5/27/2010 P Box1 F8
SP10-28-4 (green) D/L lactate hypothetical protein 0066801-66809 plasmid A 7363 13-2, 17-1 5/27/2010 P Box1 G8
Au10TR-20-36 = Sp11-JAA12-27 succinate intergenic region 0068883 plasmid A n/a 17-1 11/30/2010 P Box2 F4
Au12-316-3-9 D/L lactate putative Mrr restriction system protein 0069187 plasmid A 7364 17-1 12/13/2012 P Box3 C5
Au09MW-14-39 succinate intergenic region 0070293-70302 plasmid A n/a 13-2, 17-1 11/30/2009 P Box1 H8
Au10TR-7-184 succinate hypothetical protein 0070962 plasmid A 3910 17-1 11/30/2010 P Box2 F3
Sp11-8-68 D/L lactate hypothetical protein 0077636-0077644 plasmid A 3904 13-2, 17-1 6/13/2011 P Box 2 B8
Sp11-31-197 D/L lactate intergenic 0084516 plasmid A n/a 17-1 6/13/2011 P Box2 A8
Sp11-19-111 D/L lactate just downstream of RSP_3926 NAD-dependent epimerase/dehydratase 0101662 plasmid A n/a 17-1 6/23/2011 P Box2 B7
SP10-26-B9 D/L lactate intergenic region 0107765 plasmid A n/a 17-1 5/27/2010 P Box1 I8
Sp11-8-184 D/L lactate intergenic region (upstream of RSP_7367) 0017695 plasmid B n/a 17-1 6/23/2011 P Box2 I5
Au09TR-06-37 succinate proW, ABC Glycine betaine/L-proline transporter, inner membrane subunit 0049184-49192 plasmid B 3999 13-2, 17-1 11/30/2009 P Box1 B9
Au09MW-15-72 succinate n/a 0049200 (estimate) plasmid B n/a iPCR 11/30/2009 P Box1 C9
Sp11-15-114 D/L lactate short-chain dehydrogenase/reductase 0081992 plasmid B 3972 17-1 6/23/2011 P Box2 D6
Au10MW-10-132 succinate hypothetical protein 0085188 plasmid B 3969 17-1 11/30/2010 P Box2 I2
Au10MW-28-86 succinate hypothetical protein 0106553 plasmid B 3946 17-1 11/30/2010 P Box2 D3
Au10TR-4-37 succinate ABC sugar transporter, inner membrane subunit 0108619 plasmid B 3943 17-1 11/30/2010 P Box2 H4
SP10-BA46 D/L lactate H-NS family DNA-binding protein 0008335-8343 plasmid C 7372 13-2, 17-1 5/27/2010 P Box1 D9
Au12-316-7-237 D/L lactate intergenic 0019736 plasmid C n/a 17-1 12/13/2012 P Box3 H4
Au09MW10-127 succinate n/a 0019957-19966 plasmid C n/a 13-2, 17-1 11/30/2009 P Box1 E9
Sp11-35-109 D/L lactate intergenic 0039325-0039341 plasmid C n/a 13-2, 17-1 8/9/2011 P Box2 B9
Au09TR-27-41 succinate putative colanic acid biosynthesis acetyltransferase WcaF  0048347-48353 plasmid C 7375 13-2, 17-1 11/30/2009 P Box1 F9
Au09MW-01-17 succinate n/a 0051250 (estimate) plasmid C n/a iPCR 11/30/2009 P Box1 G9
Sp11-26-81 D/L lactate hypothetical 0052172-0052181 plasmid C 7376 13-2, 17-1 7/11/2011 P Box2 B9
Au09MW-01-67 succinate gmd, NAD-dependent, epimerase/dehydrolase 0065556-65565 plasmid C 4109 13-2, 17-1 11/30/2009 P Box1 H9
Au09MW28-15 succinate n/a 0067212 (estimate) plasmid C n/a iPCR 11/30/2009 P Box1 I9
Au12-332-18-4 D/L lactate  TraC, predicted ATPase (Walker motif) 0070992 plasmid D 4226 17-1 12/13/2012 P Box3 D3
Au12-332-14-167 D/L lactate YD repaet protein 0029801 plasmid E  4284 17-1 12/13/2012 P Box1 A8
Au12-316-19-125 D/L lactate 16SrRNA (one of two copies) chromosome 2 either 4347 or 4352 17-1 12/13/2012 P Box3 G5