Wu Lab

Recent Publications

48. Larissa V. G. Longo, Evelyn G. Goodyear , Sha Zhang , Elena Kudryashova , Jian-Qiu Wu. 2021. Involvement of Smi1 in cell wall integrity and glucan synthase Bgs4 localization during fission yeast cytokinesis. Mol. Biol. Cell. 2021 Dec 15; mbcE21040214. doi: 10.1091/mbc.E21-04-0214. Online ahead of print. PMID: 34910579.

47. Gerien K.S., Zhang S., Russell A.C., Zhu Y.-H., Purde V., Wu J.-Q. 2020. Roles of Mso1 and the SM protein Sec1 in efficient vesicle fusion during fission yeast cytokinesis. Mol. Biol. Cell. 2020 May 20:mbcE20010067. doi: 10.1091/mbc.E20-01-0067. Online ahead of print. PMID: 32432970.

46. Lucas B.E., McPherson M.T., Hawk T.M., Wilson L.N., Kroh J.M., Hickman K.G., Fitzgerald S.R., Disbennett W.M., Rollins P.D., Hylton H.M., Baseer M.A., Montgomery P.N., Wu J.-Q., Petreaca R.C. 2019. An Assay to Study Intra-Chromosomal Deletions in Yeast. Methods Protoc. 2(3):74. PMID: 31454903.

45. Okada H. *, Wloka C. *, Wu J.-Q., Bi E. 2019. Distinct roles of myosin-II isoforms in cytokinesis under normal and stressed conditions. iScience. 14:69-87. *Co-first authors. https://www.ncbi.nlm.nih.gov/pubmed/30928696

44. Liu, Y. *, N. A. McDonald*, S.M. Naegele, K.L. Gould, and J.-Q. Wu. 2019. The F-BAR domain of Rga7 relies on a cooperative mechanism of membrane binding with a partner protein during fission yeast cytokinesis. Cell Reports. 26:2540-2548. *Co-first authors. https://www.ncbi.nlm.nih.gov/pubmed/30840879

43. Liu X.M., Yamasaki A., Du X.M., Coffman V.C., Ohsumi Y., Nakatogawa H., Wu J.-Q., Noda N.N., Du L.-L. 2018. Lipidation-independent vacuolar functions of Atg8 rely on its noncanonical interaction with a vacuole membrane protein. eLIFE. 7:e41237.

42. Gerien K.S., J.-Q. Wu. 2018. Molecular mechanisms of contractile-ring constriction and membrane trafficking in cytokinesis. Biophys Rev. 10:1649-1666.

41. Zhu, Y.-H., J. Hyun, Y.-Z. Pan, J.E. Hopper, J. Rizo, J.-Q. Wu. 2018. Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis. Mol. Biol. Cell. 29:2259-2279.

40. Davidson R., J.A. Pontasch, J.-Q. Wu. 2016. Sbg1 is a novel regulator for the localization of the β-glucan synthase Bgs1 in fission yeast. PLoS One. 11: e0167043. https://www.ncbi.nlm.nih.gov/pubmed/27898700

39. Liu Y. and J.-Q. Wu. 2016. Cytokinesis: Going super resolution in live cells. Current Biology. 26:R1150-R1152. http://www.cell.com/current-biology/fulltext/S0960-9822(16)31076-4

38. Liu Y, I-J. Lee, M. Sun, C.A. Lower, K.W. Runge, J. Ma, J.-Q. Wu. 2016. Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis. Mol. Biol. Cell. 27:2528-2541. https://www.ncbi.nlm.nih.gov/pubmed/27385337

37. Wang, N., I-J. Lee, G. Rask, and J.-Q. Wu*. 2016. Roles of the TRAPP-II complex and the exocyst in membrane deposition during fission yeast cytokinesis. PLoS Biology 14:e1002437. http://www.ncbi.nlm.nih.gov/pubmed/27082518

36. Davidson R., Y. Liu, K.S. Gerien, and J.-Q. Wu*. 2016. Real-time visualization and quantification of contractile ring proteins in single living cells. Mothods in Mol. Biol. 1369:9-23. http://www.ncbi.nlm.nih.gov/pubmed/26519302

35. Sun, L., R. Guan, I-J. Lee, Y. Liu, M. Chen, J. Wang, J.-Q. Wu, Z. Chen. 2015. Mechanistic insights into the anchorage of the contractile ring by anillin and Mid1. Developmental Cell. 33:413-426. http://www.ncbi.nlm.nih.gov/pubmed/25959226

34. Xu, T., D. Vavylonis, F.-C. Tsai, G. Koenderink, W. Nie, E. Yusuf, I-J. Lee, J.-Q. Wu, and X. Huang. 2015. SOAX: A software for quantification of 3D biopolymer networks. Scientific Reports 5:9081. http://www.ncbi.nlm.nih.gov/pubmed/25765313

33. Davidson, R., D. Laporte, and J.-Q. Wu*. 2015. Regulation of Rho GEF Rgf3 by ­the arrestin Art1 in fission yeast cytokinesis. Mol. Biol. Cell. 26:453-466. PMCID: PMC4310737. http://www.ncbi.nlm.nih.gov/pubmed/25473118

32. Wang, N., M. Wang, Y.-H. Zhu, T.W. Grosel, D. Sun, D.S. Kudryashov, and J.-Q. Wu*. 2015. The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis. Mol. Biol. Cell. 26:238-255. http://www.ncbi.nlm.nih.gov/pubmed/25411334
[The paper has been chosen by the MBoC Editorial Board for Highlights. It appeared in the ASCB Newsletter and was considered for the MBoC Paper of the Year award.]

31. Coffman, V.C., I-J. Lee, and J.-Q. Wu*. 2014. Counting molecules within cells. Colloquium Series on Quantitative Cell Biology (e-book series). Biota Publishing, ISBN# 9781615046553.http://books.google.com/books?id=hfK9BAAAQBAJ

30. Wang, N., L. Lo Presti, Y.-H. Zhu, M. Kang, Z. Wu, S.G. Martin, and J.-Q. Wu*. 2014. The novel proteins Rng8 and Rng9 regulate the myosin-V Myo51 during fission yeast cytokinesis. J. Cell Biol. 205:357-375. http://www.ncbi.nlm.nih.gov/pubmed/24798735
[Highlighted by JCB at http://jcb.rupress.org/content/205/3/284.2.full].

29. Lee, I-J., N. Wang, W. Hu, K. Schott, J. Bähler, T.H. Giddings, Jr., J.R. Pringle, L.-L. Du, J.-Q. Wu*. 2014. Regulation of spindle-pole body assembly and cytokinesis by the centrin-binding protein Sfi1 in fission yeast. Mol. Biol. Cell. 25:2735-2749.

28. Deng, L, R. Kabeche, N. Wang, J.-Q. Wu, J.B. Moseley. 2014. Megadalton node assembly by binding of Skb1 to the membrane anchor Slf1. Mol. Biol. Cell. 25:2660-2668. http://www.ncbi.nlm.nih.gov/pubmed/25009287

27. Coffman, V.C*. and J.-Q. Wu*. 2014. Every laboratory with a fluorescence microscope should consider counting molecules. Mol. Biol. Cell. 25:1545-1548. Review.

26. Zhu, Y.-H. and J.-Q. Wu*. 2014. Cell-size control – complicated. Cell Cycle. 13:693-694. http://www.ncbi.nlm.nih.gov/pubmed/24526119

25. Coffman V.C., J.A. Sees, D.R. Kovar, and J.-Q. Wu*. 2013. The formins Cdc12 and For3 cooperate during contractile ring assembly in cytokinesis. J. Cell Biol. 203:101-114. http://www.ncbi.nlm.nih.gov/pubmed/24127216 [Highlighted by J. Cell Biology "In Focus" in 2013 at http://jcb.rupress.org/content/203/1/3.full].

24. Zhu Y.-H., Y.F. Ye, Z. Wu, and J.-Q. Wu*. 2013. Cooperation between Rho-GEF Gef2 and its binding partner Nod1 in the regulation of fission yeast cytokinesis. Mol. Biol. Cell. 24:3187-3204. http://www.ncbi.nlm.nih.gov/pubmed/23966468

23. Coffman, V.C. and J.-Q. Wu. 2012. Counting protein molecules using quantitative fluorescence microscopy. Trends in Biochemical Sciences. 37:499-506. http://www.ncbi.nlm.nih.gov/pubmed/22948030

22. Lee, I-J., V.C. Coffman and J.-Q. Wu. 2012. Contractile-ring assembly in fission yeast cytokinesis: recent advances and new perspectives. Cytoskeleton. 69:751-763. http://www.ncbi.nlm.nih.gov/pubmed/22887981

21. Laporte, D., N. Ojkic, D. Vavylonis, J.-Q. Wu. 2012. Alpha-Actinin and fimbrin cooperate with myosin II to organize actomyosin bundles during contractile-ring assembly. Mol. Biol. Cell. 23:3094-3110. http://www.ncbi.nlm.nih.gov/pubmed/22740629

20. Lee, I-J. and J.-Q. Wu. 2012. Characterization of Mid1 domains for targeting and scaffolding in fission yeast cytokinesis. J. Cell Sci. 125:2973-2985. http://www.ncbi.nlm.nih.gov/pubmed/22427686

19. Y.F. Ye, I-J. Lee, K.W. Runge, and J.-Q. Wu. 2012. Roles of putative Rho-GEF Gef2 in division-site positioning and contractile-ring function in fission yeast cytokinesis. Mol. Biol. Cell. 23:1181-1195. http://www.ncbi.nlm.nih.gov/pubmed/22298427

18. Valerie C. Coffman, P. Wu, M. Parthun, and J.-Q. Wu. 2011. CENP-A exceeds microtubule attachment sites in centromere clusters of both budding and fission yeast. J. Cell Biol. 195:563-572.
[Highlighted by JCB In Focus at: http://jcb.rupress.org/content/195/4/539.full].

17. P. Wu, R. Zhao, Y. Ye, and J.-Q. Wu. 2011. Roles of the DYRK kinase Pom2 in cytokinesis, mitochondrial morphology, and sporulation in fission yeast. PLoS ONE 6(12): e28000. doi:10.1371/journal.pone.0028000. http://www.ncbi.nlm.nih.gov/pubmed/22174761

16. Ojkic N., J.-Q. Wu., Vavylonis D. 2011. Model of myosin node aggregation into a contractile ring: the effect of local alignment. J. Phys Condens Matter 23:374103. http://iopscience.iop.org/0953-8984/23/37/374103/.

15. Laporte, D., V.C. Coffman, I-J. Lee, and J.-Q. Wu. 2011. Assembly and architecture of precursor nodes during fission yeast cytokinesis. J. Cell Biol. 192:1005-1021.
[Highlighted by JCB at: http://jcb.rupress.org/content/192/6/902.2.full].

14. Wu, J.-Q., Y.F. Ye, N. Wang, T.D. Pollard, and J.R. Pringle. 2010. Cooperation between the septins and the actomyosin ring and role of a cell-integrity pathway during cell division in fission yeast. Genetics. 186:897-915.

13. Laporte, D., R. Zhao, and J.-Q. Wu. 2010. Mechanisms of contractile-ring assembly in fission yeast and beyond. Seminars in Cell & Dev. Biol. 21:892-898.

12. Onishi, M., T. Koga, A. Hirata, T. Nakamura, H. Asakawa, C. Shimoda, J. Bähler, J.-Q. Wu, K. Takegawa, H. Tachikawa, J.R. Pringle, Y. Fukui. 2010. Role of septins in the orientation of forespore-membrane extension during sporulation in fission yeast. Mol. Cell Biol. 30:2057-2074. http://www.ncbi.nlm.nih.gov/pubmed/20123972

11. Thomas D. Pollard and Jian-Qiu Wu. 2010. Understanding cytokinesis: lessons from fission yeast. Nature Reviews Molecular Cell Biology. 11:149-155.

10. Valerie C. Coffman, Aaron H. Nile, I-Ju Lee, Huayang Liu, and Jian-Qiu Wu. 2009. Roles of formin nodes and myosin motor activity in Mid1p-dependent contractile ring assembly during fission yeast cytokinesis. Mol. Biol. Cell. 20:5195-5210.
[Highlighted in MBoC InCytes and ASCB Newsletter. ]

9. Vavylonis*, D, J.-Q. Wu*, S. Hao, B. O’Shaughnesy and T.D. Pollard. 2008. Assembly mechanism of the contractile ring for cytokinesis by fission yeast. Science 319:97-100.
*Co-first authors.

8. Wu, J.-Q., C. McCormick, and T. D. Pollard. 2008. Counting proteins in living cells by quantitative fluorescence microscopy with internal standards. Methods in Cell Biol. 89:253-273. http://www.ncbi.nlm.nih.gov/pubmed/19118678

7. Wu, J.-Q., V. Sirotkin, D.K. Kovar, M. Lord, C.C. Beltzner, J.R. Kuhn, and T.D. Pollard. 2006. Assembly of the cytokinetic contractile ring from a broad band of nodes in fission yeast. J. Cell Biol. 174:391-402.

6. Wu, J.-Q. and T.D. Pollard. 2005. Counting cytokinesis proteins globally and locally in fission yeast. Science 310:310-314. http://www.sciencemag.org/cgi/content/full/310/5746/310

5. Kovar, D.R., J.-Q. Wu, and T.D. Pollard. 2005. Profilin-mediated competition between capping protein and formin Cdc12p during cytokinesis in fission yeast. Mol. Biol. Cell 16:2313-2324.

4. Wu, J.-Q., J.R. Kuhn, D.R. Kovar, and T.D. Pollard. 2003. Spatial and temporal pathway for assembly and constriction of the contractile ring in fission yeast cytokinesis. Dev. Cell 5:723-734.

3. Wu, J.-Q., J. Bähler, and J.R. Pringle. 2001. Roles of a fimbrin and an a-actinin-like protein in fission yeast cell polarization and cytokinesis. Mol. Biol. Cell 12:1061-1077.

2. Hales, K.G., E. Bi, J.-Q. Wu, J.C. Adam, I.-C. Yu, and J.R. Pringle. 1999. Cytokinesis: an emerging unified theory for eukaryotes? Curr. Opin. Cell Biol. 11:717-725

1. Bähler, J.*, J.-Q. Wu*, M.S. Longtine, N.G. Shah, A. McKenzie III, A.B. Steever, A. Wach, P. Philippsen, and J.R. Pringle. 1998. Heterologous modules for efficient and versatile gene targeting in Schizosaccharomyces pombe. Yeast 14:943-951.
*Co-first authors